健康成年人唾液中胃蛋白酶含量测定在辅助检测咽喉反流中的应用研究

doi:10.3877/cma.j.issn.1674-6899.2017.01.002

中华胃食管反流病电子杂志 ›› 2017, Vol. 04 ›› Issue (01) : 7 -10. doi: 10.3877/cma.j.issn.1674-6899.2017.01.002

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健康成年人唾液中胃蛋白酶含量测定在辅助检测咽喉反流中的应用研究

李进让¹^,^†(

), 田师宇¹, 邹世桢¹, 刘涛², 于磊², 王文军³

^1. 100048　北京，海军总医院全军耳鼻咽喉头颈外科中心
^2. 050000　石家庄，河北医科大学附属第二医院耳鼻咽喉头颈外科
^3. 100084　北京，北京骐骥生物技术有限公司

收稿日期:2016-12-26 出版日期:2017-02-15
通信作者: 李进让
基金资助:
首都卫生发展科研专项基金资助(2016-2-5111)

Pepsin measurement in saliva of the healthy adults for detection of laryngopharygeal reflux

Jinrang Li¹^,^†(), Shiyu Tian¹, Shizhen Zou¹, Tao Liu², Lei Yu², Wenjun Wang³

^1. Department of Otorhinolaryngology Head and Neck Surgery, Navy General Hospital, Beijing, 100048, China
^2. Department of Otorhinolaryngology Head and Neck Surgery, The Second Affiliated Hospital of Hebei Medical University, Shijiazhuang, Hebei, 050000, China
^3. Beijing Qiji Biotechnology Co.Ltd, Beijing 100084, China

Received:2016-12-26 Published:2017-02-15
Corresponding author: Jinrang Li
About author:
corresponding author: Li Jinrang, Email: entljr@sina.com

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引用本文：

李进让, 田师宇, 邹世桢, 刘涛, 于磊, 王文军. 健康成年人唾液中胃蛋白酶含量测定在辅助检测咽喉反流中的应用研究[J/OL]. 中华胃食管反流病电子杂志, 2017, 04(01): 7-10.

Jinrang Li, Shiyu Tian, Shizhen Zou, Tao Liu, Lei Yu, Wenjun Wang. Pepsin measurement in saliva of the healthy adults for detection of laryngopharygeal reflux[J/OL]. Chinese Journal of Gastroesophageal Reflux Disease(Electronic Edition), 2017, 04(01): 7-10.

目的

初步探索健康成年人唾液中胃蛋白酶含量范围，为临床检测、诊断咽喉反流性疾病提供参考依据。

方法

2016年9月1日～9月30日在海军总医院耳鼻咽喉头颈外科门诊招募50名健康成年人作为受试者，收取受试者晨起空腹情况下黏白唾液，封存于1.5 ml无菌EP管内，并在30 min内将EP管放置于－28 ℃冰箱中冷冻，最长冷冻时间不超过2周；采用人胃蛋白酶ELISA检测试剂盒测定受试者唾液中胃蛋白酶含量。

结果

50名健康受试者晨起痰液中胃蛋白酶含量为(24.22±6.94)ng/ml，95% CI为(22.18，26.06)ng/ml。其中，男性受试者唾液中胃蛋白酶含量为(22.78±1.35)ng/ml，95% CI为(20.00，25.57)ng/ml；女性受试者唾液中胃蛋白酶含量为(25.67±1.39)ng/ml，95% CI为(22.80，28.54)ng/ml。

结论

健康成年人唾液中存在一定含量的胃蛋白酶，检测唾液胃蛋白酶含量有望用于咽喉反流性疾病的诊断。

关键词: 胃, 蛋白酶, 咽喉, 参考值

Objective

To measure the pepsin level in saliva of the healthy adults in order to find a method to diagnose the laryngopharyngeal reflux disease by measurement of the pepsin level in the saliva of patients.

Methods

From Sept.1 2016 to Sept.30, 2016 in Department of Otolaryngology Head Neck Surgery, Navy General Hospital, 50 healthy adults were randomly recruited as subjects, and the saliva was collected into 1.5ml sterile EP tubes after awaking in the morning, and the tubes were placed in a refrigerator of -28 ℃ within half an hour, meanwhile the longest freezing time was less than 2 weeks.The level of pepsin in saliva was measured by human pepsin ELISA kit.

Results

Among the 50 subjects, the average content of pepsin in saliva was(24.22±6.94)ng/ml and the 95% confidence interval was(22.18, 26.06)ng/ml.The average content of pepsin in saliva was(22.78±1.35)ng / ml and 95% confidence interval was 20.00, 25.57)ng/ml in male group.The average content of pepsin in saliva was(25.67±1.39)ng/ml and 95% confidence interval was(22.80, 28.54)ng/ml in female group.

Conclusion

The pepsin can be detected in the saliva of the healthy adults, the level of the pepsin in the saliva of the patients may be used to the diagnosis of the laryngopharyngeal reflux disease in the future.

Key words: Stomach, Pepsin, Laryngopharyngeal, Reference value

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