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中华胃食管反流病电子杂志 ›› 2026, Vol. 13 ›› Issue (01) : 16 -24. doi: 10.3877/cma.j.issn.2095-8765.2026.01.003

论著

溃疡性结肠炎中氧化应激相关基因标志物的鉴定及验证
贺欢1, 许广鑫2, 韩致毅2, 崔童2,()   
  1. 1830001 乌鲁木齐,新疆维吾尔自治区人民医院消化科
    2834000 新疆维吾尔自治区人民医院克拉玛依医院消化科
  • 收稿日期:2025-11-28 出版日期:2026-02-15
  • 通信作者: 崔童
  • 基金资助:
    克拉玛依中心医院院内项目(20230411)

Screening and identification of hub genes related to oxidative stress in ulcerative colitis based on bioinformatics analysis

Huan He1, Guangxin Xu2, Zhiyi Han2, Tong Cui2,()   

  1. 1Department of Gatroenterology, Xinjiang Uygur Autonomous Region People’s Hospital, Urumqi 830001, China
    2Department of Gatroenterology, Xinjiang Uygur Autonomous Region People’s Kalamayi Central Hospital, Kalamayi 834000, Xinjiang Uygur Autonomous Region, China
  • Received:2025-11-28 Published:2026-02-15
  • Corresponding author: Tong Cui
引用本文:

贺欢, 许广鑫, 韩致毅, 崔童. 溃疡性结肠炎中氧化应激相关基因标志物的鉴定及验证[J/OL]. 中华胃食管反流病电子杂志, 2026, 13(01): 16-24.

Huan He, Guangxin Xu, Zhiyi Han, Tong Cui. Screening and identification of hub genes related to oxidative stress in ulcerative colitis based on bioinformatics analysis[J/OL]. Chinese Journal of Gastroesophageal Reflux Disease(Electronic Edition), 2026, 13(01): 16-24.

目的

利用生物信息学方法筛选溃疡性结肠炎氧化应激相关基因的关键基因,并分析其功能,为溃疡性结肠炎的发病机制和诊治提供新思路。

方法

从基因表达数据库下载GSE179285基因表达图谱,通过GEO2R筛选差异表达基因,将差异表达基因与GeneCard数据库中筛选的氧化应激相关基因取交集,得到溃疡性结肠炎氧化应激相关差异表达基因,利用R软件对差异表达基因进行基因本体论分析及京都基因和基因组百科全书富集分析,利用Cytoscape进行蛋白互作分析网络的模块以及关键基因的筛选。收集30例溃疡性结肠炎活动期和30例健康对照组患者的结肠黏膜组织,采用实时荧光定量聚合酶链式反应(qPCR)法验证Hub基因的表达。

结果

差异表达基因与氧化应激相关基因取交集后,共筛选出95个氧化应激相关差异表达基因,在此基础上,筛选出CD44、CXCL8、缺氧诱导因子1亚基α(HIF1α)、白细胞介素1β(IL1β)、血管紧张素原(AGT)共5个关键基因。qPCR检测结果显示,与对照组相比,溃疡性结肠炎组CD44、CXCL8、HIF1α、IL1β的mRNA表达升高(P<0.0001),AGT表达无明显差异(P>0.05)。

结论

CD44、CXCL8、HIF1α、IL1β在溃疡性结肠炎中起着关键作用,可以作为溃疡性结肠炎潜在的诊断和治疗靶点。

Objective

To screen and identify key genes associated with oxidative stress in ulcerative colitis.

Methods

The GSE179285 gene expression profiles were acquired from the gene expression omnibus database. And GEO2R was used to identify differentially expressed genes. We intersected the differentially expressed genes with the oxidative stress-related genes screened in the GeneCard database to find the oxidative stress-related genes in ulcerative colitis. R software was used to conduct gene ontology and Kyoto Encyclopedia of Genes and Genomes analyses of differentially expressed genes. Module of PPI network and analysis of key genes using Cytoscape software. colon mucosal Tissue samples from 30 patients with active ulcerative colitis and healthy control subjects were collected, the expression of Hub genes was verified using quantitative real-time polymerase chain reaction (qPCR).

Results

We identified 95 differentially expressed genes in response to oxidative stress. Five key genes—CD44,CXCL8,hypoxia inducible factor 1 subunit alpha (HIF1α),interleukin-1 beta (IL1β) and angiotensinogen (AGT)—were selected as a result.qPCR analysis revealed that compared with the control group, the mRNA expression of CD44, CXCL8, HIF1α, and IL1β was elevated in the ulcerative colitis group (P<0.0001). There was no significant difference in AGT expression between the two groups (P>0.05).

Conclusion

CD44, CXCL8, HIF1α, and IL1β play critical roles in ulcerative colitis and could serve as potential diagnostic and therapeutic targets for the disease.

图1 差异表达基因筛选图1A差异表达基因的火山图;1B显著表达的前20位基因的热图。UC为溃疡性结肠炎。
图2 氧化应激相关差异表达基筛选的韦恩图注:DEGs为差异表达基因;OS为氧化应激基因。
图3 氧化应激相关差异表达GO基因富集注:GO为富集分析及基因本体论分析。
图4 氧化应激相关差异表达KEGG富集分析注:KEGG为京都基因和基因组百科全书;IL-17为白细胞介素17 ;TNF为肿瘤坏死因子;AGE-RAGE为晚期糖基化终末产物及其受体;NF-κB:核因子κB。
图5 蛋白质相互作用网络构建及功能模块分析5A蛋白质相互作用网络及功能模块分析;5B MCODE评分≥23分的功能模块。MCODE为分子复合物检测。
表2 cytoHubba 8种算法关键基因排名
表3 引物信息
图6 Hub基因表达分析CD44:分化簇44;CXCL8:趋化因子配体8;HIF1α:缺氧诱导因子1亚基α;IL1β:白细胞介素1β;AGT:血管紧张素原
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