The study on structural characteristics of intestinal flora of mice with chronic stress based on 16s rRNA sequencing

doi:10.3877/cma.j.issn.2095-8765.2022.04.002

Abstract

Abstract:

Aim

To explore the effect of stress (chronic restraint stress) on the stepwise classification and structural characteristics of intestinal flora of mice.

Methods

Eight week old SPF male Kunming mice were randomly divided into two groups: normal group (C, n=8) and chronic restraint stress group (ST, n=8); The ST group mice were restricted in the self-made restraint device for 2 h every day, and the other time the two groups of mice were free to drink and eat in the same environment. The experiment lasted for 14 days. The intestinal flora of the two groups of mice was detected by 16s rRNA high-throughput sequencing technology, and the difference of the flora composition between ST mice and C mice was compared and analyzed.

Results

The flora diversity of ST group analyzed and detected 282.00±36.69 operational taxonomic units (OTUs), that were decreased as compare to C group (353.00±26.67 OTUs, t=3.468, P=0.010); ST group Chaol index was decreased as compare to C group (357.93±45.89 vs 415.45±28.88, t=3.409, P=0.011); ST group Simpson index was increased as compare to C group (0.19±0.08 vs 0.06±0.01, t=-4.325, P=0.003), indicating that ST groups flora diversity decreased.The contents of Helicobacterceae, Lactobacilliceae, Muribagulaceae and Moraxellaceae in ST group were significantly higher than those in C group (P＜0.05); At the same time, the contents of Lachnospiraceae, Prevotella, Ruminococcaceae, Firmicutes, Bacteroides and Antibacteria in intestinal fecal flora of ST group mice decreased significantly compared with C (P＜0.05).

Conclusion

The diversity of intestinal flora in mice induced by psychological stress decreased significantly, and the distribution of intestinal flora changed significantly.

Key words: restraint stress mice, intestinal flora, 16S rRNA, diversity of flora

Maimaiti·Yisireyili, Yongkang Wang, Wubulikasimu·Wulamu, Ababokeli·Wusiman, Kelimu·Abudureyimu. The study on structural characteristics of intestinal flora of mice with chronic stress based on 16s rRNA sequencing[J]. Chinese Journal of Gastroesophageal Reflux Disease(Electronic Edition), 2022, 09(04): 181-186.

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References 20

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组别	只数	OTU	Chaol	Simpson	Shannon	Coverage
C组	8	353.00±26.67	415.45±28.88	0.06±0.01	3.69±0.68	0.998±0.0004
ST组	8	282.00±36.69	357.93±45.89	0.19±0.08	3.08±0.92	0.999±0.0005
t值		3.468	3.409	-4.325	1.269	-1.091
p值		0.010	0.011	0.003	0.245	0.311

组别	只数	OTU	Chaol	Simpson	Shannon	Coverage
C组	8	353.00±26.67	415.45±28.88	0.06±0.01	3.69±0.68	0.998±0.0004
ST组	8	282.00±36.69	357.93±45.89	0.19±0.08	3.08±0.92	0.999±0.0005
t值		3.468	3.409	-4.325	1.269	-1.091
p值		0.010	0.011	0.003	0.245	0.311

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