Effect of FuzhengXiaowei Decoction on gastric mucosal repair and Nrf2/ARE pathway in chronic atrophic gastritis rats

doi:10.3877/cma.j.issn.2095-8765.2023.02.005

Abstract

Abstract:

Objective

To study the effects of Fuzheng Xiaowei Decoction on gastric mucosal repair and nuclear tran-scription factor 2 (Nrf2)/antioxidant reaction element (ARE) pathway in chronic atrophic gastritis (CAG) rats.

Methods

Rats were randomly divided into blank control group and modeling group, and CAG model was established by 1-methyl-3-nitro-1-nitroguanidine (MNNG) combined with ethanol gavage. After modeling, rats were randomly divided into CAG group, FuzhengXiaowei decoction low-dose, medium-dose and high-dose groups and positive control group, with 10 rats/group. The rats in the low, medium and high dose groups of FuzhengXiaowei Decoction were administrated by intragastric administration of 0.74 g/kg, 1.48 g/kg and 2.22 g/kg (measured by crude drug content), respectively, while the rats in the positive control group were administrated by intragastric administration of 0.24 g/kg with vitase for 6w. The changes of body mass in each group before and after intervention were measured. The serum levels of cyclooxygenase (COX-2), inducible nitric oxide synthase (i-NOS), interleukin-6, tumor necrosis factor (TNF-α) and gastric mucosa nitric oxide (NO), nitric oxide synthase (NOS), glutathione peroxidase (GSH-Px) were used by enzyme-linked immunosorbent assay (ELISA) The levels of oxidized lipids (LPO), malondialdehyde (MDA), superoxide dismutase (SOD), hematoxylin-eosin (HE) staining were used to detect the pathological changes of gastric mucosa in rats, and the mRNA levels of Nrf2, ARE and HO-1 in gastric mucosa were determined by real-time quantitative polymerase chain reaction (RTq-PCR). The levels of Nrf2, ARE and HO-1 in gastric mucosa were determined by Western blotting.

Results

Compared with blank control group, body weight, GSH-Px and SOD levels of gastric mucosa, Nrf2, ARE and HO-1 mRNA and protein levels of gastric tissue in CAG group were significantly decreased (P<0.05). The levels of NO, NOS, LPO and MDA in gastric mucosa and the levels of COX-2, i-NOS, IL-6 and TNF-α in serum were significantly increased (P<0.05). Compared with CAG group, body weight, GSH-Px and SOD levels of gastric mucosa, Nrf2, ARE and HO-1 mRNA and protein levels of gastric tissue in FuzhengXiaowei Decoction dose groups and positive control group were significantly increased (P<0.05). The levels of NO, NOS, LPO and MDA in gastric mucosa, COX-2, i-NOS, IL-6 and TNF-α in serum of rats were significantly decreased (P<0.05), and the gastric histomathological changes of rats were significantly recovered, and with the increase of the dose of FuzhengXiaowei Decoction, the changes of various indexes were better.

Conclusion

FuzhengXiaowei Decoction can significantly inhibit the progression of chronic atrophic gastritis in rats, repair the pathological changes of gastric tissue, inhibit inflammation and oxidative stress damage, and its mechanism may be related to the regulation of Nrf2/ARE pathway.

Key words: Chronic atrophic gastritis, Fuzheng Xiaowei Decoction, The Nrf2/ARE channel

Jinying Zhang, Abula Halipu, Yanjun Zhang. Effect of FuzhengXiaowei Decoction on gastric mucosal repair and Nrf2/ARE pathway in chronic atrophic gastritis rats[J]. Chinese Journal of Gastroesophageal Reflux Disease(Electronic Edition), 2023, 10(02): 82-89.

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References 23

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基因	正向引物（5′-3′）	反向引物（5′-3′）
Nrf2	GCATAGAGCAGGACATGGAGCAAG	ACTGATGGCAGCGGAGGAAGG
ARE	TGTGGGGTCCTGTCACTATTTG	GATATCACAGCAGTAGCTGCAGG
HO-1	GCTTACACACAGAAATGGCC	AGCACCTTCCACGTTGCTG
GAPDH	AATGGTGAAGGTCGGTGTGAACG	TCGCTCCTGGAAGATGGTGATGG

基因	正向引物（5′-3′）	反向引物（5′-3′）
Nrf2	GCATAGAGCAGGACATGGAGCAAG	ACTGATGGCAGCGGAGGAAGG
ARE	TGTGGGGTCCTGTCACTATTTG	GATATCACAGCAGTAGCTGCAGG
HO-1	GCTTACACACAGAAATGGCC	AGCACCTTCCACGTTGCTG
GAPDH	AATGGTGAAGGTCGGTGTGAACG	TCGCTCCTGGAAGATGGTGATGG

组别	干预前	干预后
空白对照组	363.01±3.06	502.17±6.71
CAG组	268.19±3.46^*	340.19±2.41^*
扶正消萎汤低剂量组	261.25±3.29^*	395.68±3.42^#
扶正消萎汤中剂量组	273.41±5.24^*	449.60±2.75^#□
扶正消萎汤高剂量组	271.79±4.19^*	482.10±3.31^#△□
阳性对照组	265.88±5.33^*	493.04±4.01^#

组别	干预前	干预后
空白对照组	363.01±3.06	502.17±6.71
CAG组	268.19±3.46^*	340.19±2.41^*
扶正消萎汤低剂量组	261.25±3.29^*	395.68±3.42^#
扶正消萎汤中剂量组	273.41±5.24^*	449.60±2.75^#□
扶正消萎汤高剂量组	271.79±4.19^*	482.10±3.31^#△□
阳性对照组	265.88±5.33^*	493.04±4.01^#

组别	COX-2 （ng/ml）	i-NOS （pg/ml）	IL-6 （pg/ml）	TNF-α （pg/ml）
空白对照组	1.32±0.13	53.66±10.57	133.15±88.61	66.22±13.38
CAG组	13.73±0.62^*	336.89±3.82^*	1442.36±87.94^*	1636.±24.12^*
扶正消萎汤低剂量组	9.89±0.66^#	269.85±26.82^#	1085.42±75.95^#	1228.±47.27^#
扶正消萎汤中剂量组	6.55±0.53^#△	164.33±21.40^#△	448.53±123.29^#△	567.4±96.26^#△
扶正消萎汤高剂量组	1.45±0.52^#△□	55.13±15.50^#△□	150.86±71.52^#△□	74.93±26.13^#△□
阳性对照组	3.77±0.36^#	114.79±14.40^#	312.30±54.18^#	275.9±31.91^#

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