To explore the significance and regulatory mechanism of insulin-like growth factor 2 mRNA-binding protein 3 (IGF2BP3) expression in the senescence of colon cancer cells..

In order to facilitate comprehensive research, it is imperative to procure 50 tissue specimens from patients suffering from colon cancer, who have been attended to within our gastroenterology department between January 2022 and December 2024. Concomitantly, an equivalent number of contiguous tissue samples should be gathered.Subsequently, a subset consisting of 5 samples from each category should be earmarked for transcriptome sequencing, aimed at the identification and screening of differentially expressed genes and their corresponding proteins. HCT116 cells were incubated and passaged in an incubator, and HCT116 cell suspension was prepared for transfection. After transfection,they were divided into three groups:control group (without any treatment), IGF2BP3 interference group, and IGF2BP3 interference empty group. Cell migration assay (Trenswell) was used to detect the invasion ability of HCT116 cells, Detection of reactive oxygen species (ROS) expression levels using flow cytometry, ELISA was used to detect the content of CXCR8, transforming grouth factor-β(TGF-β), IL-17, IL-6, and soluble intercellular adhesion molecule siCAM-1 in the cell supernatant,transmission electron microscopy was used to observe the morphological changes of different groups of cells, RT-PCR and WB were used to detect the gene and protein expression levels of IGF2BP3 in each group, and immunohistochemistry was used to detect the expression of IGF2BP3 in colon cancer tissue and adjacent tissues. Expression level.

Compared with the adjacent tissues,there were 821 differentially expressed genes in the cancer tissue group,including 485 up-regulated genes and 336 down regulated genes.The five genes with the highest significant upregulation were SIM2 ANKRD18EP, IGKV1D-8, MMP3, CYP24A1, The 5 genes with the highest significant downregulation of expression are CMAHP LINC02490, G6PC1, PTPRQ, LGSN. Biological process analysis shows that these differentially expressed genes mainly function in cellular processes, biological regulation, metabolic processes, cell parts, membranes, organelles, cell parts, organelles, membranes, organelle parts, membrane parts, protein-containing complexes, extracellular region parts, and extracellular regions. KEGG pathway analysis mainly clusters the disease types in endocrine and metabolic diseases, cancer diseases, immune diseases, digestive system, immune system, endocrine system, cell growth and death, lipid metabolism, replication and repair, and other pathways. The cell experiment results showed that compared with the control group,the invasion ability of cells in the IGF2BP3 interference group was significantly reduced (P<0.01), the levels of reactive oxygen species were significantly elevated, the contents of CXCR8, TGF-β, IL-17, IL-6, and siCAM-1 were significantly reduced (all P<0.05), and the expression levels of IGF2BP3 gene and protein were significantly reduced (P<0.01).

The genes expressed in colon cancer tissues are enriched in DNA replication, cell cycle,neuroactive ligand receptor interactions, rheumatoid arthritis, and Wnt signaling pathways. The interaction between these signaling pathways and IGF2BP3 gene may regulate cellular aging, leading to changes in the tumor micro environment of colon cancer and promoting its occurrence and development.