A modified establishment method of GERD model with catheter balloon dilation

doi:10.3877/cma.j.issn.1674-6899.2015.04.011

Abstract

Abstract:

Objective

To construct a GERD animal model of anti reflux barrier defect and disturbance of gastric emptying by using balloon dilation.

Methods

12 New Zealand white rabbits were randomly divided into 2 groups(6 rabbits in each group). Balloon dilation and composite method were performed respectively.Four weeks later, the animals were killed to evaluate the modeling effect of esophagitis and stomach dynamics.

Results

The animal models were established successfully and no animal was dead.There was no significant difference in the severity of esophagitis among the groups according to the standard of histological esophagitis.After modeling, the DIS was visible, and there was no significant difference between the two groups.The model of composite method had a significant effect on gastric emptying, and the gastric emptying rate was significantly lower than that of the balloon dilatation method.

Conclusion

Models established in this study exist esophageal anti reflux barrier defect, and disturbance of gastric emptying caused by gastrointestinal motility disorder, and taking into account the role of psychological factors in the model, provide a meet clinical needs more ideal model.

Key words: Gastroesophageal reflux, Balloon dilation, Models animal, Cell gap

Qiang Wang, Bin Wang, Wei Zhang, Ming Qiu. A modified establishment method of GERD model with catheter balloon dilation[J]. Chinese Journal of Gastroesophageal Reflux Disease(Electronic Edition), 2015, 02(04): 230-232.

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References 15

1	屈坤鹏，成晓舟．我国部分地区胃食管反流病患病率的meta分析[J/CD]．中华胃食管反流病电子杂志，2015，2(1)：34-44.
2	王斌，张伟，刘晟，等．一种新型建立胃食管反流病的动物模型的方法：导尿管球囊扩张术[J/CD]．中华胃食管反流病电子杂志，2014，1(1)：52-55.
3	Kandulski A, Jechorek D, Caro C, et al.Histomorphological differentiation of non-erosive reflux disease and functional heartburn in patients with PPI-refractory heartburn[J]. Aliment Pharmacol Ther, 2013, 38(6): 643-651.
4	魏兰福，邹百仓，魏睦新．莪术对实验性功能性消化不良大鼠胃排空的影响[J]．南京医科大学学报：自然科学版，2003，23(4)：350-352.
5	Rey E, García-Alonso M, Moreno-Ortega M, et al.Influence of psychological distress on characteristics of symptoms in patients with GERD: the role of IBS comorbidity[J]. Dig Dis Sci, 2009, 54(2): 321-327.
6	Nahata M, Muto S, Oridate N, et al.Impaired ghrelin signaling is associated with gastrointestinal dysmotility in rats with gastroesophageal reflux disease[J]. Am J Physiol Gastrointest Liver Physiol, 2012, 303(1): G42-53.
7	仇明，张伟．胃排空障碍与胃食管反流病[J/CD]．中华胃食管反流病电子杂志，2015，2(1)：1-3.
8	王瑞华，欧阳钦，王立东．三种不同术式反流性食管炎动物模型的建立[J]．郑州大学学报：医学版，2006，41(1)：115-117.
9	徐志诚，胡廷泽，刘文英，等．反流性食管炎动物模型制备的新方法[J]．中国修复重建外科杂志，2004，18(4)：288-290.
10	Hu Y, Xu X, Xu L, et al.Dilated intercellular space in the larynx and esophagus of a rabbit reflux model[J]. Auris Nasus Larynx, 2013, 40(4): 379-382.
11	郑朝旭，林伟斌，余俊峰，等，反流性食管炎大鼠模型的制备：不同管径内支架支撑幽门及前胃结扎[J]．中国组织工程研究与临床修复，2010，14(20)：3705-3708.
12	Asaoka D, Nagahara A, Oguro M, et al.Establishment of a reflux esophago-laryngitis model in rats[J]. Dig Dis Sci, 2011, 56(5): 1299-1308.
13	刘晶，李峰，唐旭东，等．功能性消化不良动物模型及其在中医研究中的应用概述[J]．环球中医药，2013，6(12)：955-958.
14	陈晨，全俊，李曼蓉，等．功能性消化不良重叠肠易激综合征动物模型的建立[J]．实验动物科学，2013，30(4)：18-21.
15	Liu LS, Winston JH, Shenoy MM, et al.A rat model of chronic gastric sensorimotor dysfunction resulting from transient neonatal gastric irritation[J]. Gastroenterology, 2008, 134(7): 2070-2079.

?	只数	食管炎指数(0/1/2/3)	pH<4时间比例(%)	反流次数	时间>5 min的反流次数	细胞间隙增宽指数	胃排空率	慢波频率的变异系数	变异节律的指数
球囊扩张法	6	1/1/4/0	13.66±6.80	36.00±7.97	4.33±3.14	1.83±0.75	34.00±8.99	16.00±5.48	10.67±6.38
复合法	6	0/2/3/1	13.33±6.47	34.67±16.75	3.50±3.39	1.50±0.84	13.50±6.74	28.67±8.61	27.33±11.24
P值	?	0.497	0.932	0.864	0.668	0.485	0.001	0.012	0.01

?	只数	食管炎指数(0/1/2/3)	pH<4时间比例(%)	反流次数	时间>5 min的反流次数	细胞间隙增宽指数	胃排空率	慢波频率的变异系数	变异节律的指数
球囊扩张法	6	1/1/4/0	13.66±6.80	36.00±7.97	4.33±3.14	1.83±0.75	34.00±8.99	16.00±5.48	10.67±6.38
复合法	6	0/2/3/1	13.33±6.47	34.67±16.75	3.50±3.39	1.50±0.84	13.50±6.74	28.67±8.61	27.33±11.24
P值	?	0.497	0.932	0.864	0.668	0.485	0.001	0.012	0.01

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