To discuss the diet induced obesity(DIO)on gastroesophageal changes in mice for investigating the role of obesity in the pathogenesis of gastroesophageal reflux disease, and provide with simple and effective experimental basis.

16 male KunMing mice were randomly divided into two groups which are DIO and normal control(NC)group using Microsoft Office Exel.Mice in DIO group were given to high-fat diet, while NC group was given normal diet.After 8 weeks of experiment, glucose tolerance test(GTT)and insulin tolerance test(ITT)were performed firstly, and esophagus, stomach and blood sample of all mice were taken for further detecting the parameters and analyzing histological changes in tissues through HE staining.

75% of mice in experimental group improved to DIO and body weight increment of mice in DIO group is(23.16±2.82)g, significantly higher than that of NC group(13.40±1.31)g(P<0.05). GTT and ITT showed that DIO group happened impaired glucose tolerance and insulin resistance.The result of ELISA indicated that the level of total cholesterol and low density lipoprotein in DIO group were significantly higher than those of NC mice(P<0.05), but the level of triglyceride has no significant difference between two groups(P>0.05). Histological changes appeared in DIO group include hyperplasia and infiltration of inflammatory cells in gastroesophageal mucosa.

DIO model in mice has imitated the process of obesity happens in humans, and it′s appropriate to evaluate obesity induced GERD through this model.Our study provides with not only simple, but also effective experimental basis which has referential value on the research of investigating the role of obesity in the pathogenesis of GERD.