切换至 "中华医学电子期刊资源库"
高级检索
中华胃食管反流病电子杂志

中华胃食管反流病电子杂志 ›› 2018, Vol. 05 ›› Issue (02) : 56 -60. doi: 10.3877/cma.j.issn.1674-6899.2018.02.003

所属专题： 文献；

论著

慢性束缚应激对小鼠胃食管的影响
买买提·依斯热依力1, 吾布力卡斯木·吾拉木2, 艾克拜尔·艾力1, 李义亮3, 阿孜古丽·阿力木江4, 阿丽叶古丽·艾皮热1, 巴突尔·艾克木2, 蒋媛3, 赵新胜2, 阿卜杜萨拉木·阿卜杜合力力2, 克力木·阿不都热依木1,†()   
  1. 1. 830001　乌鲁木齐，新疆维吾尔自治区人民医院普外微创研究所；830001　乌鲁木齐，新疆维吾尔自治区人民医院微创，疝和腹壁外科
    2. 830054　乌鲁木齐，新疆医科大学
    3. 830001　乌鲁木齐，新疆维吾尔自治区人民医院微创，疝和腹壁外科
    4. 830001　乌鲁木齐，新疆维吾尔自治区人民医院妇产科门诊
  • 收稿日期:2018-02-18 出版日期:2018-05-15
  • 通信作者: 克力木·阿不都热依木
  • 基金资助:
    新疆自治区自然科学基金(2018D01C134)

Effect of chronic restraint stress on gastroesophageal histological changes in mice

Yisireyili Maimaiti·1, Wulamu Wubulikasimu·2, Aili Aikebaier·1, yiliang Li3, Alimujiang Aziguli·4, Aipire Aliyeguli·1, Aikemu Batuer·2, Yuan Jiang3, Xinsheng Zhao2, Abuduhelili Abudusalamu·2, Abudureyimu Kelimu·1,†()   

  1. 1. Research Institute of General and Minimally Invasive Surgery; Department of Minimally Invasive Surgery, Hernia and Abdominal Wall Surgery, People′s Hospital of Xinjiang Uygur Autonomous Region, Urumqi 830001, China
    2. Graduate School of Xinjiang Medical University, Xinjiang Uygur Autonomous Region, Urumqi 830054, China
    3. Department of Minimally Invasive Surgery, Hernia and Abdominal Wall Surgery, People′s Hospital of Xinjiang Uygur Autonomous Region, Urumqi 830001, China
    4. Department of Obstetrics and Gynecology Clinic, People′s Hospital of Xinjiang Uygur Autonomous Region, Urumqi 830001, China
  • Received:2018-02-18 Published:2018-05-15
  • Corresponding author: Abudureyimu Kelimu·
  • About author:
    Corresponding author: Kelimu·Abudureyimu, Email:
全文 ( ) 下载PDF ( ) 导出引用
引用本文：

买买提·依斯热依力, 吾布力卡斯木·吾拉木, 艾克拜尔·艾力, 李义亮, 阿孜古丽·阿力木江, 阿丽叶古丽·艾皮热, 巴突尔·艾克木, 蒋媛, 赵新胜, 阿卜杜萨拉木·阿卜杜合力力, 克力木·阿不都热依木. 慢性束缚应激对小鼠胃食管的影响[J]. 中华胃食管反流病电子杂志, 2018, 05(02): 56-60.

Yisireyili Maimaiti·, Wulamu Wubulikasimu·, Aili Aikebaier·, yiliang Li, Alimujiang Aziguli·, Aipire Aliyeguli·, Aikemu Batuer·, Yuan Jiang, Xinsheng Zhao, Abuduhelili Abudusalamu·, Abudureyimu Kelimu·. Effect of chronic restraint stress on gastroesophageal histological changes in mice[J]. Chinese Journal of Gastroesophageal Reflux Disease(Electronic Edition), 2018, 05(02): 56-60.

目的

探讨心理应激在胃食管反流病(GERD)发生、发展中的作用，并为束缚应激模型的成功建立提供参考指标。

方法

20只雄性SPF级昆明小鼠随机分2组(每组10只)，即慢性束缚应激(CRS)组和正常对照(NC)组。CRS组小鼠每天在自制式束缚器中限制活动2 h，其余时间2组小鼠在相同环境中自由饮水摄食，实验持续14 d。实验结束后通过葡萄糖耐量试验(GTT)、胰岛素耐受试验(ITT)、小鼠胃食管组织及血清，检测相关指标确认该模型建立成功与否，同时在光学显微镜下观察胃食管黏膜组织学改变。

结果

CRS组小鼠体重增加量为(8.6±3.1)g，明显低于NC组小鼠(12.5±3.0)g，差异有统计学意义(P<0.05)；胰岛素耐受试验(ITT)结果显示，CRS组小鼠出现胰岛素抵抗现象；ELISA实验显示CRS组小鼠HPA轴相关激素水平明显高于NC组，差异有统计学意义(P<0.05)。CRS组小鼠胃食管黏膜可见充血、水肿及炎症细胞的浸润。

结论

CRS可影响机体，引起GERD相关组织病理学变化。为阐述心理因素在GERD中的作用机制研究提供简单有效并且具有借鉴意义的实验基础。

关键词: 慢性束缚应激, 胃食管反流病, 小鼠模型
Objective

To discuss the effect of chronic restraint stress(CRS)on gastroesophageal histological changes in mice for investigating the role of psychological factor in the pathogenesis of gastroesophageal reflux disease, and provide with referential parameters for successful establishment of CRS model.

Methods

20male KunMing mice were randomly divided into two groups which are CRS and normal control(NC)group.Mice in CRS group were submitted to partial restraint stress for 2 h per day.After 14 days of restraint stress, glucose tolerance test(GTT)and insulin tolerance test(ITT)were performed firstly, and esophagus, stomach and blood sample of all mice were taken for further detecting the parameters and analyzing histological changes in tissues through HE staining.

Results

Body weight increment of mice in CRS group is significantly lower than that of NC group(P<0.05). ITT shows that CRS group happened insulin resistance.The result of ELISA indicated that the level of hormones related to HPA axis in CRS group was significantly higher than those of NC mice(P<0.05). Histological changes appeared in CRS group include hyperemia, edema and infiltration of inflammatory cells in gastroesophageal mucosa.

Conclusion

CRS obviously affects physiological activities and may result in GERD-related histopathological changes in stomach and esophagus.Our study provides with not only simple, but also effective experimental basis which has referential value on the research of investigating the role of psychological factor in the pathogenesis of GERD.

Key words: Chronic restraint stress, Gastroesophageal reflux disease, Mice
图1 慢性束缚应激模型的建立过程
图2 CRS对HPA轴相关激素的影响
图3 CRS对糖耐量和胰岛素耐受的影响
图4 CRS对胃、食管黏膜的影响的病理图像(×200)
1
Sifrim D, Zerbib F. Diagnosis and management of patients with reflux symptoms refractory to proton pump inhibitors[J]. Gut, 2012, 61(9)：1340.
2
Fass R, Naliboff BD, Fass SS, et al.The effect of auditory stress on perception of intraesophageal acid in patients with gastroesophageal reflux disease[J]. Gastroenterology, 2008, 134(3)：696-705.
3
Song EM, Jung HK, Ji MJ.The association between reflux esophagitis and psychosocial stress[J]. Dig Dis Sci, 2013, 58(2)：471-477.
4
Jung YS, Kim MY, Lee HS, et al.Effect of DA-9701, a novel prokinetic agent, on stress-induced delayed gastric emptying and hormonal changes in rats[J]. Neurogastroenterol Motil, 2013, 25(3)：254-259.
5
El-Serag HB, Sweet S, Winchester CC, et al.Update on the epidemiology of gastro-oesophageal reflux disease：a systematic review[J]. Gut, 2014, 63(6)：871-880.
6
Uchida Y, Takeshita K, Yamamoto K, et al.Stress augments insulin resistance and prothrombotic state：role of visceral adipose-derived monocyte chemoattractant protein-1[J]. Diabetes, 2012, 61(6)：1552-1561.
7
Yisireyili M, Hayashi M, Wu H, et al.Xanthine oxidase inhibition by febuxostat attenuates stress-induced hyperuricemia, glucose dysmetabolism, and prothrombotic state in mice[J]. Sci Rep, 2017, 7(1)：1266.
8
Elserag HB.Time trends of gastroesophageal reflux disease：a systematic review[J]. Clin Gastroenterol Hepatol, 2007, 5(1)：17-26.
9
Dubois RW, Aguilar D, Fass R, et al.Consequences of frequent nocturnal gastro‐oesophageal reflux disease among employed adults：symptom severity, quality of life and work productivity[J]. Aliment Pharmacol Ther, 2007, 25(4)：487-500.
10
Zhang L, Tu L, Chen J, et al.Health-related quality of life in gastroesophageal reflux patients with noncardiac chest pain：Emphasis on the role of psychological distress[J]. World J Gastroenterol, 2017, 23(1)：127-134.
11
Okuyama M, Takaishi O, Nakahara K, et al.Associations among gastroesophageal reflux disease, psychological stress, and sleep disturbances in Japanese adults[J]. Scand J Gastroenterol, 2017, 52(1)：44-49.
12
克力木·阿不都热依木, 张成, 汪忠镐. 胃食管反流病与食管裂孔疝外科临床研究现状与争议[J/CD]. 中华胃食管反流病电子杂志, 2014, 1(1)：4-6.
13
巴突尔·艾克木, 克力木·阿不都热依木. 炎症因子重新揭示胃食管反流病发病之谜[J/CD]. 中华胃食管反流病电子杂志, 2016, 3(3)：135-138.
14
Mastracci L, Spaggiari P, Grillo F, et al.Microscopic esophagitis in gastro-esophageal reflux disease：individual lesions, biopsy sampling, and clinical correlations[J]. Virchows Archiv, 2009, 454(1)：31.
15
Yisireyili M, Takeshita K, Hayashi M, et al.Dipeptidyl peptidase-IV inhibitor alogliptin improves stress-induced insulin resistance and prothrombotic state in a murine model[J]. Psychoneuroendocrinology, 2016, 73：186.
16
Gadek-Michalska A, Spyrka J, Rachwalska P, et al.Influence of chronic stress on brain corticosteroid receptors and HPA axis activity[J]. Pharmacol Rep, 2013, 65(5)：1163-1175.
17
Jeong JY, Lee DH, Kang SS.Effects of chronic restraint stress on body weight, food intake, and hypothalamic gene expressions in mice[J]. Endocrinol Metab(Seoul), 2013, 28(4)：288-296.
18
Belda X, Fuentes S, Daviu N, et al.Stress-induced sensitization：the hypothalamic-pituitary-adrenal axis and beyond[J]. Stress, 2015, 18(3)：269-279.
19
Fiocca R, Mastracci L, Riddell R, et al.Development of consensus guidelines for the histologic recognition of microscopic esophagitis in patients with gastroesophageal reflux disease：the Esohisto project[J]. Hum Pathol, 2010, 41(2)：223-231.
[1] 贾卓奇, 周维茹, 张勇, 张广健, 付军科. 达·芬奇机器人与腹腔镜食管裂孔疝修补术的对比研究[J]. 中华疝和腹壁外科杂志(电子版), 2023, 17(04): 410-414.
[2] 李亮, 洪楚原, 李明哲, 郝腾飞, Chongkuo Zi. 胃食管结合部抗反流机制探讨[J]. 中华疝和腹壁外科杂志(电子版), 2023, 17(01): 6-8.
[3] 唐英俊, 李华娟, 王赛妮, 徐旺, 刘峰, 李羲, 郝新宝, 黄华萍. 人脐带间充质干细胞治疗COPD小鼠及机制分析[J]. 中华肺部疾病杂志(电子版), 2023, 16(04): 476-480.
[4] 张继舜, 陈思旭, 钱洁, 尚占民, 郝建宇. 平均夜间基线阻抗对食管外症状胃食管反流病的临床诊断价值[J]. 中华胃肠内镜电子杂志, 2023, 10(02): 97-102.
[5] 王擎, 王冠峰, 陈星. 胃食管阀瓣的Hill分级在胃食管反流病中的应用价值[J]. 中华胃肠内镜电子杂志, 2023, 10(02): 125-130.
[6] 陆洪鹏, 袁芯, 郑树灏, 刘怡, 徐磊. 慢性咳嗽与胃食管反流病：双向两样本孟德尔随机化研究[J]. 中华胃食管反流病电子杂志, 2023, 10(02): 59-64.
[7] 范勇, 张利昉, 杨美琳. 改良经口内镜下贲门缩窄术治疗胃食管反流病效果分析[J]. 中华胃食管反流病电子杂志, 2023, 10(02): 65-68.
[8] 文明, 熊英, 艾克拜尔·艾力, 克力木·阿不都热依木. 质子泵抑制剂治疗期间焦虑对胃食管反流病疗效的影响[J]. 中华胃食管反流病电子杂志, 2023, 10(02): 90-95.
[9] 李鹏, 刘国祥, 李汝红. 经口内镜下贲门缩窄术治疗胃食管反流病的研究进展[J]. 中华胃食管反流病电子杂志, 2023, 10(02): 96-99.
[10] 买买提·依斯热依力, 尹强, 尹海龙, 董雨微, 王永康, 克力木·阿不都热依木, 阿吉艾克拜尔·艾萨. 传统医药治疗胃食管反流病的研究进展[J]. 中华胃食管反流病电子杂志, 2023, 10(02): 100-104.
[11] 闫晶, 蒋媛, 买买提·依斯热依力, 王俭, 王永康, 阿巴伯克力·乌斯曼, 王志, 克力木·阿不都热依木. 膈肌生物反馈训练对胃食管反流病的效果分析[J]. 中华胃食管反流病电子杂志, 2023, 10(01): 50-55.
[12] 张晓贝, 王伟刚, 李晓龙, 易风俊, 俞永江. 幽门螺旋杆菌感染与不同亚型胃食管反流病的相关性[J]. 中华胃食管反流病电子杂志, 2023, 10(01): 1-5.
[13] 张高松, 杨美琳, 袁莉, 俞炎波, 石琳妮. 内镜下贲门缩窄术对改善胃食管反流病患者生活质量的观察[J]. 中华胃食管反流病电子杂志, 2023, 10(01): 18-21.
[14] 李赞林, 马建惠, 王志. 腹腔镜袖状胃切除术对胃食管反流病疗效的系统评价与分析[J]. 中华胃食管反流病电子杂志, 2023, 10(01): 22-30.
[15] 伍振鹏, 乔钰涵, 向林, 江云颂, 彭居正, 吴丽娜, 程吕佳, 关炳生, 庄子康, 杨景哥. 腹腔镜袖状胃切除术中胃窦切缘与幽门的距离对术后胃食管反流病及减重效果的影响[J]. 中华肥胖与代谢病电子杂志, 2023, 09(01): 4-11.
阅读次数
全文


摘要

版权所有 中华医学会 中华医学电子音像出版社有限责任公司  京ICP备14006079号-1  网络出版服务许可证：(署)网出证(京)字第075号