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中华胃食管反流病电子杂志

中华胃食管反流病电子杂志 ›› 2021, Vol. 08 ›› Issue (02) : 55 -60. doi: 10.3877/cma.j.issn.2095-8765.2021.02.002

论著

p38 丝裂原活化蛋白激酶、上皮细胞间质转化在胃食管反流病及食管腺癌组织中的表达及其临床意义
钟江利1, 王红1,†(), 赵凌苇1   
  1. 1. 563000　贵州，遵义医科大学附属医院消化内科
  • 收稿日期:2020-12-16 出版日期:2021-05-15
  • 通信作者: 王红

Expression and clinical significance of p38 MAPK、EMT in Gastroesophageal reflux disease and adenocarcinoma tissue

Jiangli Zhong1, Hong Wang1,†(), Lingwei Zhao1   

  1. 1. Department of Gastroenterology, Affiliated Hospital of Zunyi Medical University, Guizhou 563000, China
  • Received:2020-12-16 Published:2021-05-15
  • Corresponding author: Hong Wang
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钟江利, 王红, 赵凌苇. p38 丝裂原活化蛋白激酶、上皮细胞间质转化在胃食管反流病及食管腺癌组织中的表达及其临床意义[J]. 中华胃食管反流病电子杂志, 2021, 08(02): 55-60.

Jiangli Zhong, Hong Wang, Lingwei Zhao. Expression and clinical significance of p38 MAPK、EMT in Gastroesophageal reflux disease and adenocarcinoma tissue[J]. Chinese Journal of Gastroesophageal Reflux Disease(Electronic Edition), 2021, 08(02): 55-60.

目的

探讨p38丝裂原活化蛋白激酶（MAPK）、E-cadherin、Vimentin蛋白在胃食管反流病及食管腺癌组织中的表达及其意义。

方法

收集2017年1月至2019年4月遵义医科大学附属医院病理科42例胃食管反流病患者蜡块标本（来源于消化内科内镜活检患者）为胃食管反流病组、2013年1月至2019年4月9例食管腺癌患者标本（来源于胸外科行食管腺癌切除术患者）为食管腺癌组、9例食管腺癌癌旁组织标本（来源于食管腺癌患者癌旁黏膜组织）为癌旁组。运用免疫组化法检测p38 MAPK、E-cadherin、Vimentin在3组患者中的表达情况，分析上述指标与胃食管反流病、食管腺癌的关系。

结果

在正常食管组织、胃食管反流病、食管腺癌组织中，p38 MAPK、Vimentin蛋白的表达呈逐渐上升趋势，E-cadherin蛋白的表达呈下降趋势。

结论

p38 MAPK、Vimentin、E-cadherin的异常表达可能参与了胃食管反流病及食管腺癌的发病。

关键词: 胃食管反流病, p38 MAPK, E-cadherin, Vimentin
Objective

To explore the Expression and significance of p38 mitogen-activated protein kinase(MAPK)、E-cadherin, Vimentin protein in gastroesophageal reflux disease and esophageal adenocarcinoma tissues.

Methods

Collect from January 2017 to April 2019 Wax block specimens from 42 patients(undergoing endoscopic biopsy in Gastroenterology) in Pathology Department of Zunyi Medical University were gastroesophageal reflux disease group, 9 patients with esophageal adenocarcinoma (undergoing esophageal adenocarcinoma resection) from January 2013 to April 2019 were esophageal adenocarcinoma group, nine esophageal adenocarcinoma adjacent tissue specimens (derived from the adjacent mucosal tissue of esophageal adenocarcinoma patients) were the paracancerous group. Immunohistochemical method was used to detect the expression of p38 MAPK, E-cadherin, and Vimentin in the three groups of patients, and the relationship between the above indicators and gastroesophageal reflux disease and esophageal adenocarcinoma was analyzed.

Results

In paracancerous tissues of esophageal adenocarcinoma, gastroesophageal reflux disease tissues, esophageal adenocarcinoma tissues, p38 MAPK and Vimentin are expressed in a gradually increasing trend, while E-cadherin is expressed in a gradually decreasing trend.

Conclusion

The abnormal expression of p38 MAPK、E-cadherin, Vimentin may be involved in the pathogenesis of gastroesophageal reflux disease and esophageal adenocarcinoma.

Key words: Gastroesophageal reflux disease, p38 MAPK, E-cadherin, Vimentin
图1 -p38 MAPK在3组中的表达（×400）
表1 p38 MAPK、E-cadherin、Vimentin在食管腺癌癌旁组织、胃食管反流病组织、食管腺癌组织中的阳性率（%）
指标 癌旁组（9例） 胃食管反流病组（42例） 食管腺癌组（9例） χ2值 P值
p38 MAPK 1（11.1） 25（59.5） 8（88.9） 11.351 0.002*
E-cadherin 8（88.9） 32（76.2） 2（22.2） 10.549 0.004*
Vimentin 1（11.1） 29（69.0） 8（88.9） 12.870 0.001*
图2 -E-cadherin在3组中的表达（×400）
图3 -E-Vimentin在3组中的表达（×400）
表2 p38 MAPK、E-cadherin、Vimentin在胃食管反流病的表达与临床特征之间的关系
临床特征 例数 p38 MAPK P值 E-cadherin P值 Vimentin P值
+ - + - + -
性别 0.666 0.666 0.654

男性

 27 23 4 20 7 18 9

女性

 15 12 3 12 3 11 4
年龄 0.374 0.608 0.355

≥60岁

 14 7 7 10 4 8 6

＜60岁

 28 18 10 22 6 20 8
Hp感染 0.735 0.548 0.974

有

 16 9 7 13 3 11 5

无

 26 16 10 19 7 18 8
GERD类型 0.028* 0.469 0.019*

RE

 21 9 12 17 4 11 10

BE

 21 16 5 15 6 18 3
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